Manganese-Enhanced cardiac MRI (MEMRI) tracks long-term in vivo survival and restorative benefit of transplanted human Amnion-Derived Mesenchymal Stem Cells (hAMSC) after porcine ischemia-reperfusion injury
نویسندگان
چکیده
Manganese-Enhanced cardiac MRI (MEMRI) tracks long-term in vivo survival and restorative benefit of transplanted human Amnion-Derived Mesenchymal Stem Cells (hAMSC) after porcine ischemia-reperfusion injury Rajesh Dash, Paul J Kim, Yuka Matsuura, Xiaohu Ge, Fumiaki Ikeno, Jennifer K Lyons, Ngan F Huang, Scott Metzler, Patricia Nguyen, Shahriar Heidary, Marie-Claude Parent, Tomoaki Yamamoto, John Cooke, Pilar Ruiz-Lozano, Robert C Robbins, Joseph C Wu, Michael V McConnell, Alan Yeung, Phillip Harnish, Phillip C Yang
منابع مشابه
Manganese-Enhanced Magnetic Resonance Imaging Enables In Vivo Confirmation of Peri-Infarct Restoration Following Stem Cell Therapy in a Porcine Ischemia–Reperfusion Model
BACKGROUND The exact mechanism of stem cell therapy in augmenting the function of ischemic cardiomyopathy is unclear. In this study, we hypothesized that increased viability of the peri-infarct region (PIR) produces restorative benefits after stem cell engraftment. A novel multimodality imaging approach simultaneously assessed myocardial viability (manganese-enhanced magnetic resonance imaging ...
متن کاملManganese-enhanced MRI enables longitudinal tracking of transplanted stem cell viability in the murine myocardium
Background Stem cell therapy in the heart is limited by an inability to track transplanted cell survival. To address this limitation, we used human amnion-derived mesenchymal stem cells (hAMSCs), which exhibit longer in vivo survival, and Manganese (Mn2+)-Enhanced MRI (MEMRI), which enters live stem cells to augment T1 signal. We tested Mn2+ pre-labeling of hAMSCs in vitro and whether MEMRI wou...
متن کاملTime series analysis of in vivo cardiac MRI-PET image fusion of the human amniotic mesenchymal stem cell (hAMSC) engraftment
Methods Porcine ischemia reperfusion (IR) injury model was employed to assess the successful stem cell engraftment signal by manganese enhanced MRI (MEMRI). To validate the MEMRI signal, hAMSCs were transduced by PET reporter gene (RG) utilizing the herpes simplex virus-thymidine kinase transgene construct, which is expressed only in engrafted cells to trap 18F-FHBG radiotracer. One week after ...
متن کاملManganese-enhanced MRI detects live human amnion-derived mesenchymal stem cells in vivo after transplantation and restoration of myocardial function in a pig ischemia-reperfusion injury model
Manganese-enhanced MRI detects live human amnion-derived mesenchymal stem cells in vivo after transplantation and restoration of myocardial function in a pig ischemia-reperfusion injury model Rajesh Dash, Ildiko Toma, Fumiaki Ikeno, Jennifer K Lyons, Shahriar Heidary, Marie-Claude Parent, INing E Wang, Xiaohu Ge, Jaehoon Chung, Justin Lam, Paul J Kim, Kaori Nakagawa, Svetlana Lyalina, Grace Do,...
متن کاملDual manganese-enhanced and delayed gadolinium-enhanced MRI detects myocardial border zone injury in a pig ischemia-reperfusion model.
BACKGROUND Gadolinium (Gd)-based delayed-enhancement MRI (DEMRI) identifies nonviable myocardium but is nonspecific and may overestimate nonviable territory. Manganese (Mn(2+))-enhanced MRI (MEMRI) denotes specific Mn(2+) uptake into viable cardiomyocytes. We performed a dual-contrast myocardial assessment in a porcine ischemia-reperfusion (IR) model to test the hypothesis that combined DEMRI a...
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